Principles DNase I footprinting assay is an in vitro method to identify the specific site of DNA binding proteins. It not only finds the target protein that binds to specific DNA, but also identify which sequence the target protein is bound. This technique can be used to study interactions between proteins and DNA both outside and within cells. The protein binds to the DNA fragment, protecting the binding site from cleavage by the DNase I. The fragments of the DNA molecule are left after cleavage (also known as the "footprinting", and thus its sequence can be determined. On the autoradiogram of the polyacrylamide electrophoresis gel, there is no radiolabeled band corresponding to the site of protein binding. Footprinting assay is specific, accurate positioning, and widely used. more athttps://www.creativebiomart.net/resource/principle-protocol-dnase-i-footprinting-377.htm ...read more

Background and Principle Many cell related life processes involve the interaction between DNA and proteins. With the development of recombinant DNA technology, a wide range of critical genes have been isolated. Now the key issue is to reveal how environmental factors and developmental signals controlling gene transcription. Therefore, it is necessary to identify and analyze DNA elements involved in the regulation of gene expression, and to isolate and identify these cis-element-specific protein factors. All these studies involve in the interaction between DNA and proteins. Methylation interference assay is a method of identifying which regions of DNA strands bind to specific proteins by methylation modification of nucleotides. The rationale is that dimethyl sulfate (DMS) methylates the exposed guanine residue (G) in DNA, the piperidine in turn chemically cleaves methylated guanine residues. Methylation interference assay can be used to study the relationship between transcription factors and guanine residues in DNA binding sites. It is also an effective complement to DNase I footprinting assay to identify the precise location of DNA-protein interactions. more at https://www.creativebiomart.net/resource/principle-protocol-methylation-interference-assay-378.htm ...read more

Background The discovery of non-coding RNA makes RNA become the focus of life science research once again. Because RNA is an unstable biological macromolecule, most RNAs need to be bound to a specific RNA-binding protein to form an RNA / protein complex in order to be stable in cells. Furthermore, dynamic association between RNA and RNA-binding proteins throughout and accompanies the entire life cycle of RNA transcriptional synthesis, processing and modification, intracellular trafficking and localization, functional development and degradation. Therefore, take advantage of RNA-binding proteins to isolate or discover functional RNA molecules is an indispensable research method in the field of RNA research. RNA binding protein immunoprecipitation (RIP) assay utilizes antibodies against the target protein to precipitate the corresponding RNA-protein complexes. The RNA bound to the complex can be validated by q-PCR or sequencing analysis after isolation and purification. RIP is a technique to study the combination of RNA and protein in cells. It is a powerful tool to understand the dynamic process of post-transcriptional regulation of the network, which can help us discover the regulatory targets of miRNAs. Workflow more athttps://www.creativebiomart.net/resource/principle-protocol-rna-binding-protein-immunoprecipitation-rip-380.htm ...read more